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51315-6081-Visualization and Quantification of Biofilm Removal for the Mitigation of MIC

Picture for Visualization and Quantification of Biofilm Removal for the Mitigation of MIC
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Product Number: 51315-6081-SG
ISBN: 6081 2015 CP
Author: Timothy Tidwell
Publication Date: 2015
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$20.00
$20.00
Biofilms are the predominant antagonists when it comes to microbiologically influenced corrosion (MIC). The removal of this biofilm community is crucial to mitigating the risk of a corrosion failure due to MIC. Despite this there are few current techniques that can accurately address whether a biocide treatment is effectively removing the biofilm or simply lowering its metabolic activity thereby leaving the infrastructure intact for new microbes to more easily attach to surfaces. We have developed a technique utilizing Confocal Laser Scanning Microscopy to determine the efficiency of biofilm removal of commonly used oilfield biocides using thickness and volume calculations. Volume measurements allow us to quantify the ability of the biocide to penetrate throughout the biofilm rather than only seeing a reduction in overall thickness which can be influenced by a number of factors. Our results not only show the mechanism of how a biofilm can be removed under various conditions but we also show how insufficient dosing can contribute to a more rapid biofilm recovery and actually result in an exacerbated MIC risk.
Biofilms are the predominant antagonists when it comes to microbiologically influenced corrosion (MIC). The removal of this biofilm community is crucial to mitigating the risk of a corrosion failure due to MIC. Despite this there are few current techniques that can accurately address whether a biocide treatment is effectively removing the biofilm or simply lowering its metabolic activity thereby leaving the infrastructure intact for new microbes to more easily attach to surfaces. We have developed a technique utilizing Confocal Laser Scanning Microscopy to determine the efficiency of biofilm removal of commonly used oilfield biocides using thickness and volume calculations. Volume measurements allow us to quantify the ability of the biocide to penetrate throughout the biofilm rather than only seeing a reduction in overall thickness which can be influenced by a number of factors. Our results not only show the mechanism of how a biofilm can be removed under various conditions but we also show how insufficient dosing can contribute to a more rapid biofilm recovery and actually result in an exacerbated MIC risk.
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51317--9604-Mitigation of Severe Pitting Corrosion Caused by MIC in a CDC Biofilm Reactor

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To study the effect of repeated biocide treatments to mitigate microbiologically influenced corrosion (MIC), we used a Center for Disease Control (CDC) biofilm reactor to generate and remediate corrosive biofilms on carbon steel coupons grown from a produced water sample from a salt water disposal (SWD).
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51318-11616-High Concentration Biocide Treatment of an Oil Pipeline

Product Number: 51318-11616-SG
Author: Zach Broussard / Timothy J Tidwell / Renato De Paula / Victor Keasler
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An oil transmission pipeline in the Eagle Ford area was being treated with 150ppm of active biocide based on a five percent water hold up but good control of the microbial population was not being maintained.

 
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