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51317--9604-Mitigation of Severe Pitting Corrosion Caused by MIC in a CDC Biofilm Reactor

Picture for Mitigation of Severe Pitting Corrosion Caused by MIC in a CDC Biofilm Reactor
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To study the effect of repeated biocide treatments to mitigate microbiologically influenced corrosion (MIC), we used a Center for Disease Control (CDC) biofilm reactor to generate and remediate corrosive biofilms on carbon steel coupons grown from a produced water sample from a salt water disposal (SWD).

Product Number: 51317--9604-SG
ISBN: 9604 2017 CP
Author: Timothy Tidwell
Publication Date: 2017
Industry: Science of Corrosion
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Microbially influenced corrosion (MIC) of metallic iron surfaces can be attributed to two different mechanisms. Chemical MIC (cMIC) occurs when biological activities modify the local microenvironment by generating acid or excreting H2S which induces a corrosive process on the metal surface. Electrochemical MIC (eMIC) however occurs when corrosion is propagated via direct electron uptake from a metallic surface. In this study we used a once-through biofilm reactor to generate corrosive biofilms from an oilfield produced water sample from West Texas. Initially we screened two biocidal products on coupons from the biofilm reactor to determine which chemistry would best kill and remove the biofilm from the surface. Then we used the best performing biocide to batch treat the biofilm reactor every week for eight weeks to determine how quickly the biofilms would recover and if biocide treatment would mitigate the severe corrosion. During the first three weeks ATP quantification showed a consistent 90% percent reduction in sessile microbial activity after a four hour biocide batch and a subsequent rebound once the biocide was eliminated from the reactor while DNA sequencing showed that the treatments managed to permanently eliminate Methanocalculus a hydrogenotrophic methanogen linked to eMIC from biofilm reactor bulk fluids. Results from this study indicate that in addition to reducing the overall and corrosion-related microbial populations pitting corrosion was significantly reduced with a weekly batch treatment of the top-performing biocide.

Key words: Microbially Influenced Corrosion, MIC, eMIC, Biocides, DNA, ATP, Methanogens

Microbially influenced corrosion (MIC) of metallic iron surfaces can be attributed to two different mechanisms. Chemical MIC (cMIC) occurs when biological activities modify the local microenvironment by generating acid or excreting H2S which induces a corrosive process on the metal surface. Electrochemical MIC (eMIC) however occurs when corrosion is propagated via direct electron uptake from a metallic surface. In this study we used a once-through biofilm reactor to generate corrosive biofilms from an oilfield produced water sample from West Texas. Initially we screened two biocidal products on coupons from the biofilm reactor to determine which chemistry would best kill and remove the biofilm from the surface. Then we used the best performing biocide to batch treat the biofilm reactor every week for eight weeks to determine how quickly the biofilms would recover and if biocide treatment would mitigate the severe corrosion. During the first three weeks ATP quantification showed a consistent 90% percent reduction in sessile microbial activity after a four hour biocide batch and a subsequent rebound once the biocide was eliminated from the reactor while DNA sequencing showed that the treatments managed to permanently eliminate Methanocalculus a hydrogenotrophic methanogen linked to eMIC from biofilm reactor bulk fluids. Results from this study indicate that in addition to reducing the overall and corrosion-related microbial populations pitting corrosion was significantly reduced with a weekly batch treatment of the top-performing biocide.

Key words: Microbially Influenced Corrosion, MIC, eMIC, Biocides, DNA, ATP, Methanogens

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51317--9615-MIC Prevention in Oxidizer Treated Water Systems: A Study on Relative Reaction Rates

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ISBN: 9615 2017 CP
Author: Ramakrishnan Balasubramanian
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In this paper, we will present a study that is aimed at understanding the relative rates of reactions between oxidizers and bacteria, iron sulfide, H2S, other oxidizable compounds present in produced waters and the overall impact on metal corrosion.
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Microbiologically influenced corrosion has been attributed to the activity of sulfate reducing and acid producing bacteria. Advances in DNA isolation and sequencing have revealed that these classes of bacteria often represent only a small portion of the corrosive microbial population present in the oil and gas environment.
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ISBN: 7347 2016 CP
Author: sarah eisenlord
Publication Date: 2016
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We describe the advancement of an activity-based quantitative polymerase chain reaction (qPCR) assay which can distinguish live from dead corrosion influencing microorganisms in oil and gas pipeline environments. We discuss the limitations and possible future optimization methods for Propidium monazide-qPCR techniques in the industry.
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