This standard describes field test methods that are useful for estimating bacterial populations, including sessile bacterial populations, commonly found in oilfield systems. The described test methods are those that can be done on site and that require a minimum of laboratory equipment or supplies. The described test methods are not the only methods that can be used, but they are methods that have been proven to be useful in oilfield situations. This standard is meant to be used by technical field and service personnel, including those who do not necessarily have extensive or specific training in microbiology. However, because microbiology is a specialized field, some pertinent and specific technical information and explanation are provided to the user. Finally, the implications of the results obtained by these test methods are beyond the scope of this standard. The interpretation of the results is site- and system specific and may require more expertise than can be provided by this standard. This standard is loosely based on a document produced by the former Corrosion Engineering Association (CEA). CEA operated in the United Kingdom under the auspices of NACE and the Institute of Corrosion (ICorr).This NACE International standard was developed by NACE Task Group T-1C-21 under the direction of Unit Committee T-1 Con Corrosion Monitoring in Petroleum Production and is issued under the auspices of Group Committee T-1 on Corrosion Control in Petroleum Production.

1.1.1 This standard describes field test methods for estimating bacterial populations commonly found in oilfield systems. Although these techniques have been successful in the oil field, they are not the only methods that are used. It is not the intent of this standard to exclude additional techniques that can be proven useful. However, caution should be exercised with any technique that is at variance from those outlined here.

1.1.2 This standard deals only with bacteria and does not consider other organisms that may be found in oilfield fluids, such as phytoplankton (algae), protozoa, or fungi. In addition, these methods are not applicable to marine organisms such as zooplankton (copepods).

1.1.3 Because effective sampling is essential to any successful analysis, emphasis is given to sampling methods that are suitable for use in oilfield conditions.

 Historical Document 1994