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In-situ electrochemical techniques were used to investigate the effect of sulfate-reducing bacteria specie Desulfovibrio alaskensis AL1 colonization and its metabolic products on carbon steel corrosion. Open circuit potential (OCP) measurements showed a slight potential shift toward more positive values appearing just after microbial inoculations.
In-situ electrochemical techniques were used to investigate the effect of sulfate-reducing bacteria specie Desulfovibrio alaskensis AL1 colonization and its metabolic products on carbon steel corrosion. Open circuit potential (OCP) measurements showed a slight potential shift toward more positive values appearing just after microbial inoculations. Conversely, a stable OCP was observed in abiotic conditions. Comparative observations between test systems (with permitted and restricted surface colonization) and microbial specie suggest the OCP shift is attributed to a fine balance between biogenic sulfide (a result of microbial metabolic activity), and the ferrous component of steel surface. The analysis of electrochemical impedance spectroscopy (EIS) data suggested different properties of formed surface layers, depending on the nature of the environment. Alteration of surface electric properties have been attributed to complex deposits comprising extracellular polymeric substance (EPS) embedding microbial cells and/or biomineralization/corrosion products that synergistically transformed the electrochemical surface interface and increased steel resistance to corrosion.
Key words: downloadable, carbon steel, Desulfovibrio alaskensis AL1, corrosion, OCP, EIS
We describe the advancement of an activity-based quantitative polymerase chain reaction (qPCR) assay which can distinguish live from dead corrosion influencing microorganisms in oil and gas pipeline environments. We discuss the limitations and possible future optimization methods for Propidium monazide-qPCR techniques in the industry.
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