AMPP Store - Products tagged with 'qpcr'

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51316-7194-Monitoring and Risk Assessment of Microbiological Influenced Corrosion in Offshore Pipelines

Product Number: 51316-7194-SG

ISBN: 7194 2016 CP

Author: Uffe Thomsen

Publication Date: 2016

$20.00

The monitoring program used in the Danish Sector of the North Sea to manage microbiologically influenced corrosion (MIC) risk assessment for seven pipelines. Quantitative data on microbial activity was obtained from pigging debris using real-time polymerase chain reaction of MIC-causing microorganisms.

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51316-7278-Molecular Microbiological Methods to Investigate Microbial Influenced Corrosion in Fully Integrated Kraft Pulp and Paper Mills

Product Number: 51316-7278-SG

ISBN: 7278 2016 CP

Author: Dora Ogles

Publication Date: 2016

$20.00

Corrosion in modern paper mills accounts for 30+% of maintenance expenses. Molecular microbiological methods (MMM): • Quantitative polymerase chain reaction (qPCR) and QuantArray were employed to examine MIC at four paper mills each with unique process characteristics and construction materials in the affected areas.

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51318-10902-Quantifying Sulfur Oxidizing Bacteria using qPCR

Product Number: 51318-10902-SG

Author: Scott Leleika / Amanda Harmon / Sarah Eisenlord

Publication Date: 2018

$20.00

We have developed a quantitative polymerase chain reaction (qPCR) assay to detect and quantify sulfur oxidizing bacteria (SOB) through the amplification of the soxB subunit of the thiosulfate-oxidizing gene complex. SOB populations have been linked to the corrosion of concrete and steel.

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51318-10982-Analyzing Pig Returns from a Subsea Pipeline for MIC: Sampling and Testing Challenges

Product Number: 51318-10982-SG

Author: John J. Kilbane II Ph.D.

Publication Date: 2018

$20.00

This manuscript provides case study data from subsea crude oil pipelines that addresses the questions of how to obtain the best quality samples from pig returns for microbiological testing, and what are the relative merits of different test methodologies.

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51318-11103-A combination of qPCR RT-qPCR and NGS provides a new tool for assessment of MIC risk in pipelines

Product Number: 51318-11103-SG

Author: Uffe Sognstrup Thomsen / Søren Kahns / Mike Christian Oehler

Publication Date: 2018

$20.00

Sampling of pigging debris was performed from three multiphase pipelines that previously were exposed to microbiologically influenced corrosion (MIC) due to high abundances of sulfate reducing prokaryotes (SRP) and methanogens.

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51318-11662-DNA sequencing of oilfield samples: impact of protocol choices on the microbiological conclusions

Product Number: 51318-11662-SG

Author: Renato De Paula / Cruz St Peter / Ian Alex Richardson / Jep Bracey / Ed Heaver / Kathleen Duncan / Mary Eid / Ralph Tanner

Publication Date: 2018

$20.00

In this study, we retrieved multiple samples from several wells in an onshore oilfield and submitted them for 16S rDNA taxonomic analysis in two different laboratories. The results showed significant differences between laboratories.

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Can We Expand ATP Assay and Molecular Techniques to Measure Microbes on Surfaces and Under Deposits?

Product Number: 51317--9414-SG

ISBN: 9414 2017 CP

Author: Kim Dockens

Publication Date: 2017

$20.00

This paper compares planktonic and sessile counts using a variety of testing methods (including culture media, ATP assay and nucleic acid analysis). Data will be presented from a series of case studies including both lab work and field assessments.

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Case Study for MIC Evaluation and Mitigation in Two Argentinian Oilfields

Product Number: 51317--9476-SG

ISBN: 9476 2017 CP

Author: Emerentiana Sianawati

Publication Date: 2017

$20.00

A biocide efficacy study was conducted using field water samples and associated indigenous microorganisms as the test inocula. Thirteen biocide systems were evaluated to determine an excellent choice for disinfecting (rapid kill), to alleviate bio-burden and result in longer term protection.

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Challenges in Microbial Monitoring of an Oil-Water Separation Facility

Product Number: MECC23-20123-SG

Author: Alexander Grigoryan

Publication Date: 2023

$20.00

Water-handling oil producing facilities often become target for microbial contamination because treated waters are not sterile – they are inhabited by various microorganisms and contain sufficient inorganic and organic nutrients to support microbial growth. The bacterial contamination and bioburden are to extravagate easily if environmental conditions in the facilities, for instance, moderate temperature (<45C) and salinity (<50 g/l TDS), favor microorganisms. Growing bacterial population distributes along the system and forms biofilms on the surfaces of pipelines, valves, vessels, tanks, etc. Such spreading of free-floating (planktonic) and sessile (biofilm) bacteria in industrial systems is referred to as biofouling.

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MIC Risk Assessment of Sea Water Injection Systems: the Genome Centric Approaches

Product Number: 51324-20699-SG

Author: Nazima Habibi; Saif Uddin; Nasreem Abdul Razzack; Abdul Hameed Al-Hashem; Moavin Islam

Publication Date: 2024

$40.00

Microorganisms are a nuisance in water injection systems. Dense bacterial growth within the pipelines is the originator of reservoir souring, biofouling, biocorrosion and biodegradation of the infrastructure, resulting in huge expenses on the operational and capital costs. Molecular methods are superior tools for identifying microbial populations in the system and mapping the threat level. qPCR is a technology that allows us to quantify the absolute concentration of different bacterial and archaeal groups present in samples collected in the field. This analysis can be done on-site or in-laboratory. Another technology called next-generation sequencing (NGS), can dig deep into the interesting samples to determine the identity and relative populations of specific bacteria and archaea down to the genus or species level that are present. However, both of these techniques cannot differentiate between viable and non-viable communities. Recently RNA based estimations have been observed as a method to quantify the living microbial communities. Coupling RT-PCR, qPCR and NGS together gives a complete picture of what is happening and thus opens avenues for more effective monitoring and treatment.

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Microbial and Compositional Analyses of Crude Pipeline Samples

Product Number: MECC23-20101-SG

Author: Fawziah S. Alajmi

Publication Date: 2023

$20.00

Microbiologically influenced corrosion (MIC) has been an emerging concern in the oil and gas industry. Pipeline networks of different services, such as sour crude, sweet crude, water, and gas are subjected to various corrosion types, including MIC from microbial activities present within these systems. Such microbial activities can hinder the pipeline integrity and lead to metal deterioration.

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Microbial Corrosion Diagnosis Using Molecular Microbiology Methods: Case Studies

Product Number: MPWT19-14427

Author: Xiangyang Zhu, Abdullah H. Wadei

Publication Date: 2019

$0.00

Microbiologically influenced corrosion (MIC) is one of the leading causes of equipment and pipeline failure in oil and gas industries. Cost-effective MIC management requires routine monitoring of microbial activities, periodic assessment of microbial risks in various operational systems, and accurate diagnosis of MIC failure. Traditionally, MIC diagnosis has been dependent on cultivation-based methods by inoculating liquid samples containing live bacteria into selective growth media, followed by incubation at a certain temperature for a pre-determined period of time. The conventional culturing techniques have been reported to severely underestimate the size of the microbial populations related to metal corrosion, among many inherited weaknesses of these techniques. As a result, accurate diagnosis of MIC failure is challenging because the conventional techniques often fail to provide a critical piece of evidence required for a firm diagnosis, i.e., the presence of corrosion-causing microorganisms in the failed metal samples. In this paper, we described applications of molecular microbiology methods in diagnosing MIC in a crude oil pipeline and crude processing facility. Molecular microbial analyses have provided a solid piece of evidence to firmly diagnose the MIC in a crude oil flow line, a stagnant bypass spool, and a global valve bypass pipe. The presence of a high number of corrosion-related microorganisms in upstream pipelines poses a high risk to downstream crude processing facilities for microbial contamination and corrosion failure in these facilities. An effective MIC management program should include routine monitoring of microbial activities and risk assessment, and effective mitigation program, such as scraping and biocide treatments.

Products tagged with 'qpcr'

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