Menu
Search
Filters
Close
Menu

51314-4477-Microbially Influenced Corrosion

Picture for Microbially Influenced Corrosion
Available for download
Product Number: 51314-4477-SG
ISBN: 4477 2014 CP
Author: Kerry Sublette
Publication Date: 2014
$0.00
$20.00
$20.00
Molecular Analysis of Resin-Embedded Corrosive Biofilm LayersMicrobiologically influenced corrosion (MIC) is a serious problem that impacts nearly all industries and exacts a severe toll in terms of operating costs loss of production deterioration of capital equipment and the consequences of corrosion related failures. While a proactive step monitoring for MIC associated microorganisms is often hindered by the fact that sampling methods may not capture key members of the microbial community involved in MIC (sampling biases) and current reliance on conventional culture-dependent methods which may underestimate and oversimplify the problem (cultivation bias). Molecular biological tools (MBTs) based on analysis of DNA extracted directly from field samples circumvent the limitations of culture-depend methods. However sampling biases must also be addressed in order to ensure that molecular analyses truly provide more accurate and comprehensive MIC evaluation. Eckert et al (2003) have developed a method for embedding corrosion products and biofilms formed on corrosion coupons in a cured resin conserving their spatial orientation for a variety of analytical techniques. In the current study a procedure was developed for DNA extraction from discrete sections of the resin embedded biofilm. Quantitative polymerase chain reaction (qPCR) was then used to quantify total bacteria and specific microorganisms commonly implicated in MIC. The development of the extraction procedure along with improvements in sectioning will permit the DNA based analyses (qPCR microarrays and even high-throughput sequencing) for the most direct characterization of microbial communities of corrosive biofilms. 
Molecular Analysis of Resin-Embedded Corrosive Biofilm LayersMicrobiologically influenced corrosion (MIC) is a serious problem that impacts nearly all industries and exacts a severe toll in terms of operating costs loss of production deterioration of capital equipment and the consequences of corrosion related failures. While a proactive step monitoring for MIC associated microorganisms is often hindered by the fact that sampling methods may not capture key members of the microbial community involved in MIC (sampling biases) and current reliance on conventional culture-dependent methods which may underestimate and oversimplify the problem (cultivation bias). Molecular biological tools (MBTs) based on analysis of DNA extracted directly from field samples circumvent the limitations of culture-depend methods. However sampling biases must also be addressed in order to ensure that molecular analyses truly provide more accurate and comprehensive MIC evaluation. Eckert et al (2003) have developed a method for embedding corrosion products and biofilms formed on corrosion coupons in a cured resin conserving their spatial orientation for a variety of analytical techniques. In the current study a procedure was developed for DNA extraction from discrete sections of the resin embedded biofilm. Quantitative polymerase chain reaction (qPCR) was then used to quantify total bacteria and specific microorganisms commonly implicated in MIC. The development of the extraction procedure along with improvements in sectioning will permit the DNA based analyses (qPCR microarrays and even high-throughput sequencing) for the most direct characterization of microbial communities of corrosive biofilms. 
Product tags
Also Purchased
Picture for Failure Analysis of Reactor Effluent Air Cooler (REAC) in a Hydrocracker Unit
Available for download

Failure Analysis of Reactor Effluent Air Cooler (REAC) in a Hydrocracker Unit

Product Number: 51314-3730-SG
ISBN: 3730 2014 CP
Author: Surajit Ghosal
Publication Date: 2014
$0.00

A major fire in a Reactor Effluent Air Cooler (REAC) of the Hydrocracker Unit (HCU). Visual inspection was made on the failed portion. Metallurgical investigation, macrostructural and microstructural analysis, Scanning Electron Microscopy, and Energy Dispersive x-ray Spectroscopy was conducted on the failed air cooler.
Picture for Corrosion of Carbon Steel and Alloys in Ammonium Chloride Salt
Available for download

Corrosion of Carbon Steel and Alloys in Ammonium Chloride Salt

Product Number: 51314-4007-SG
ISBN: 4007 2014 CP
Author: Kazuhiro Toba
Publication Date: 2014
$20.00

This study was conducted to determine the root cause of aggressive corrosion due to wet NH4Cl salt deposition in reactor effluent streams in hydroprocessing units. In addition corrosion resistance and behavior of carbon steel and alloys in high-concentration NH4Cl solutions were investigated.
Picture for Advances in Materials and Corrosion Control in Fossil and Alternative Fuel Combustion and Conversion
Available for download

51314-4459-Advances in Materials and Corrosion Control in Fossil and Alternative Fuel Combustion and Conversion

Product Number: 51314-4459-SG
ISBN: 4459 2014 CP
Author: Georg Andersohn
Publication Date: 2014
$20.00
Categories
Industry
Recently viewed
Popular tags
View all

Association for Materials Protection and Performance

© AMPP All Rights Reserved.
Membership Terms of Content Use
Contact Customer Support