98527 ASPECTS OF COOLING TOWER BIOCIDES AND PROTOZOA

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Product Number: 51300-98527-SG

ISBN: 98527 1998 CP

Author: S.G. Berk, R.J. Ashburn, R.S. Ting

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Previous work has shown that certain cooling tower amoebae and ciliated protozoa are resistant to several cooling tower biocides, even at the manufacturer’s recommended dosages. For the present study, an Acanthamoeba species was isolated from a cooling tower in Australia. Suspensions of the trophozoites (feeding stages) were exposed to isothiazolones. Cysts were tested separately. The minimum lethal concentration (MLC) for trophozoites was between 31-62 ppm of the biocide product, which is slightly less than the MLC for an amoebae species from the United States; and cyst forms were twofold more resistant than those of the U.S. species, with a MLC of 62,500 ppm. A ciliate and an amoeba species were also exposed to bromochlorodimethylhydantoin. The MLC for the ciliate species was 1 ppm of the biocide product, and the MLC was 30-40 ppm for the amoeba trophozoites. Since amoebae can expel vesicles containing live Legionella, experiments were conducted to determine whether exposure of Acanthamoebu polyphaga to biocides influenced release of such potentially infectious particles. Vesicle release was not inhibited by any of the three biocides: quaternary ammonium compounds (QACs), isothiazolones, and a thiocarbamate compound. These results suggest that amoebae from various sources are resistant to recommended levels of biocides, and the amoebae may continue to release potentially infectious vesicles in the presence of biocides. Keywords: biocides, amoeba, cooling tower, protozoa