The effect of chemical cleaning using an Electric Power Research Institute-Steam Generator Owners Group (EPRISGOG) iron removal solvent on the surface roughness of the secondary side of fouled and microfouled Alloy 800 steam generator tubes waa characterized using Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The latter technique was used to quantify the surface roughness of the tubes before and after chemical cleaning. The surface roughness as a function of exposure time to the iron removal solvent was determined. It was found that the surface roughness of the fouled tube and the unfouled tube was slightiy increased by chemical cleaning. The degree of roughening for the unfouled tube paralleled the corrosion of the tube surface. The surface roughness changes for the fouled tube indicated an isotropic dissolution of the oxide. Keywords: Surface Roughness, chemical cleaning, atomic force microscopy, SEM.