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10255 Simultaneous Online Monitoring of SRB Activity and Corrosion Rate

Picture for 10255 Simultaneous Online Monitoring of SRB Activity and Corrosion Rate
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Product Number: 51300-10255-SG
ISBN: 10255 2010 CP
Author: Tesfa Haile, Reeta D. Sooknah, Sankara Papavinasam, Doug Gould and Orlando Dinardo
Publication Date: 2010
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$20.00
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The performance of a sulfide oxidase (SO) based biosensor for online monitoring of sulfatereducing bacteria (SRB) activity was evaluated by correlating the electrochemical response of the biosensor to the biogenic sulfide concentration from a SRB-reactor. Facultative Actinomycete strain FR- 3 was used to produce the SO enzyme; the enzyme was immobilized on a graphite paste along with a cofactor 7, 7, 8, 8-tetracyanoquinodimethane (TCNQ) or 1, 1’-dimethylferrocene (DMF). The ferrocenebased biosensor responded linearly to aqueous sulfide concentration up to 180 mg/L [S-2 (mg/L) = 8.3× I (µA/cm2)]. When TCNQ was used as a co-factor, the biosensor responded linearly up to biogenic sulfide concentration of 15 mg/L [S-2 (mg/L) = 0.57 × I (µA/cm2)].

In order to determine the relationship between biogenic sulfide production and corrosion rate, a carbon steel electrode was immersed in the SRB reactor. The corrosion rate was monitored using the electrochemical polarization resistance technique. The corrosion rate peaked during the exponential growth phase of the bacteria and declined afterwards, probably due to the formation of a biofilm on the biosensor and electrode surface as well as an FeS layer on the surface of carbon steel.

Keywords - Biosensor, enzyme electrode, SRB, MIC, electrochemistry, online monitoring
The performance of a sulfide oxidase (SO) based biosensor for online monitoring of sulfatereducing bacteria (SRB) activity was evaluated by correlating the electrochemical response of the biosensor to the biogenic sulfide concentration from a SRB-reactor. Facultative Actinomycete strain FR- 3 was used to produce the SO enzyme; the enzyme was immobilized on a graphite paste along with a cofactor 7, 7, 8, 8-tetracyanoquinodimethane (TCNQ) or 1, 1’-dimethylferrocene (DMF). The ferrocenebased biosensor responded linearly to aqueous sulfide concentration up to 180 mg/L [S-2 (mg/L) = 8.3× I (µA/cm2)]. When TCNQ was used as a co-factor, the biosensor responded linearly up to biogenic sulfide concentration of 15 mg/L [S-2 (mg/L) = 0.57 × I (µA/cm2)].

In order to determine the relationship between biogenic sulfide production and corrosion rate, a carbon steel electrode was immersed in the SRB reactor. The corrosion rate was monitored using the electrochemical polarization resistance technique. The corrosion rate peaked during the exponential growth phase of the bacteria and declined afterwards, probably due to the formation of a biofilm on the biosensor and electrode surface as well as an FeS layer on the surface of carbon steel.

Keywords - Biosensor, enzyme electrode, SRB, MIC, electrochemistry, online monitoring
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