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10249 Effects of Nitrate on Bacterial Communities in an Oil Field Environment

Picture for 10249 Effects of Nitrate on Bacterial Communities in an Oil Field Environment
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Product Number: 51300-10249-SG
ISBN: 10249 2010 CP
Author: Abdulmohsen A. Al-Humam, Tony Rizk, Jan A. Sunner and Iwona B. Beech
Publication Date: 2010
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The study aimed to determine whether nitrate-utilizing sulfate-reducing bacteria (SRB) thrive in the Hawtah oil field produced water re-injection system (PWRI). Bacterial populations were recovered through enrichments in selective growth media from samples collected at different locations in PWRI. Characterization of purified Hawtah field SRB isolates using 16S rRNA gene-based phylogeny, revealed the presence of an isolate, coded DD-H SRB, showing a high 16S rRNA sequence similarity (99%) with Desulfovibrio desulfuricans spp. desulfuricans. The change from sulfate to nitrate reduction was tested using the DD-H SRB isolate and a “wild” Hawtah SRB enrichment in the laboratory batch culture experiments. Control culture comprised a tri-species mixture of pure Desulfovibrio strains. Both, the DD-H SRB isolate and the control SRB culture, when exposed to a gradually decreasing concentration of sulfate and a concurrently increasing concentration of nitrate over a 40-day period, changed slowly from sulfate to nitrate respiration. Upon inoculation into a sulfate-free medium, fast nitrate reduction was observed. In the “wild” Hawtah field SRB population, the transition to nitrate reduction was faster. The functional gene aprA, coding for adenosine-5-phosphosulfate (APS)-reductase enzyme, which is found in all known SRB genera, was present in all tested cultures grown with nitrate as a sole electron acceptor.

Keywords: Sulfate-reducing bacteria, nitrate treatment, oil-field microbiology, aprAB functional gene, nitrate reduction, Desulfovibrio desulfuricans.
The study aimed to determine whether nitrate-utilizing sulfate-reducing bacteria (SRB) thrive in the Hawtah oil field produced water re-injection system (PWRI). Bacterial populations were recovered through enrichments in selective growth media from samples collected at different locations in PWRI. Characterization of purified Hawtah field SRB isolates using 16S rRNA gene-based phylogeny, revealed the presence of an isolate, coded DD-H SRB, showing a high 16S rRNA sequence similarity (99%) with Desulfovibrio desulfuricans spp. desulfuricans. The change from sulfate to nitrate reduction was tested using the DD-H SRB isolate and a “wild” Hawtah SRB enrichment in the laboratory batch culture experiments. Control culture comprised a tri-species mixture of pure Desulfovibrio strains. Both, the DD-H SRB isolate and the control SRB culture, when exposed to a gradually decreasing concentration of sulfate and a concurrently increasing concentration of nitrate over a 40-day period, changed slowly from sulfate to nitrate respiration. Upon inoculation into a sulfate-free medium, fast nitrate reduction was observed. In the “wild” Hawtah field SRB population, the transition to nitrate reduction was faster. The functional gene aprA, coding for adenosine-5-phosphosulfate (APS)-reductase enzyme, which is found in all known SRB genera, was present in all tested cultures grown with nitrate as a sole electron acceptor.

Keywords: Sulfate-reducing bacteria, nitrate treatment, oil-field microbiology, aprAB functional gene, nitrate reduction, Desulfovibrio desulfuricans.
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