Menu
Search
Filters
Close
Menu

Use of a Methodological Panel for Microbial Diagnostics Biocide Selection and Application in an Oil Shale Field

Picture for Use of a Methodological Panel for Microbial Diagnostics Biocide Selection and Application in an Oi
Available for download

An oil shale field was found to exhibit classic signs of a heavy microbial burden. Data generated by this exhaustive testing and screening were used to influence biocide choice and applications in the field. Impact of biocides on the field microbial characteristics are described and discussed.

Product Number: 51317--9019-SG
ISBN: 9019 2017 CP
Author: Jennifer Fichter
Publication Date: 2017
Industries: Chemical Inhibitors , Oil and Gas Production
$0.00
$20.00
$20.00

An oil shale field was found to exhibit classic signs of a heavy microbial burden including incidences of hydrogen sulfide production down hole and surface microbially influenced corrosion downhole pump and surface equipment fouling and fracturing fluid and drilling mud degradation. Over 130 samples including formation core material drilling muds fracturing fluid source waters production well samples samples collected from failed pipe surfaces and samples from salt water disposal facilities were collected in a comprehensive survey. Microbial activity was measured in parallel using four different bacterial quantification methods: 1. Traditional MPN culture-based assay for SRB APB GHB (aka "bug bottles) 2. Direct visualization and counting bacterial cells utilizing live/dead staining coupled to flow cytometry 3. An ATP-based assay for metabolically active cells and 4. A hydrolase-based assay for metabolically active cells. Additionally the microbial populations of some samples were characterized genetically using 16S amplicon metagenomics. Biocide selection tests were performed with frac water sources and a drilling mud sample. The survey results suggested that the bacterial activity could be attributed primarily to introduced water sources as opposed to indigenous formation microbes. Data generated by this exhaustive testing and screening were used to influence biocide choice and applications in the field. Impact of biocides on the field microbial characteristics are described and discussed.

 

Key words: corrosion, microbial monitoring, biocides, APB, acid producing bacteria, SRB, sulfate

reducing bacteria, biofilm, biofouling, bug bottles, flow cell cytometer, ATP, hydrolase, sulfidogenesis,

16S amplicon metagenomics

Key words: corrosion, microbial monitoring, biocides, APB, acid producing bacteria, SRB, sulfate reducing bacteria, biofilm, biofouling, bug bottles, flow cell cytometer, ATP, hydrolase, sulfidogenesis, 16S amplicon metagenomics

 

An oil shale field was found to exhibit classic signs of a heavy microbial burden including incidences of hydrogen sulfide production down hole and surface microbially influenced corrosion downhole pump and surface equipment fouling and fracturing fluid and drilling mud degradation. Over 130 samples including formation core material drilling muds fracturing fluid source waters production well samples samples collected from failed pipe surfaces and samples from salt water disposal facilities were collected in a comprehensive survey. Microbial activity was measured in parallel using four different bacterial quantification methods: 1. Traditional MPN culture-based assay for SRB APB GHB (aka "bug bottles) 2. Direct visualization and counting bacterial cells utilizing live/dead staining coupled to flow cytometry 3. An ATP-based assay for metabolically active cells and 4. A hydrolase-based assay for metabolically active cells. Additionally the microbial populations of some samples were characterized genetically using 16S amplicon metagenomics. Biocide selection tests were performed with frac water sources and a drilling mud sample. The survey results suggested that the bacterial activity could be attributed primarily to introduced water sources as opposed to indigenous formation microbes. Data generated by this exhaustive testing and screening were used to influence biocide choice and applications in the field. Impact of biocides on the field microbial characteristics are described and discussed.

 

Key words: corrosion, microbial monitoring, biocides, APB, acid producing bacteria, SRB, sulfate

reducing bacteria, biofilm, biofouling, bug bottles, flow cell cytometer, ATP, hydrolase, sulfidogenesis,

16S amplicon metagenomics

Key words: corrosion, microbial monitoring, biocides, APB, acid producing bacteria, SRB, sulfate reducing bacteria, biofilm, biofouling, bug bottles, flow cell cytometer, ATP, hydrolase, sulfidogenesis, 16S amplicon metagenomics

 

Product tags
Also Purchased
Picture for Understanding Insulation Chemistry Proven to Inhibit Corrosion Under Insulation (CUI)
Available for download

Understanding Insulation Chemistry Proven to Inhibit Corrosion Under Insulation (CUI)

Product Number: 51317--8876-SG
ISBN: 8876 2017 CP
Author: David Shong
Publication Date: 2017
$20.00

Since 2002, a corrosion inhibiting chemistry package has been an integral part of two specific industrial insulations. This paper explains, at a molecular level, how this package engages a two-pronged defense (physical coating and pH buffering) against CUI.
Picture for 08658 Use of Microbiocides in Barnett Shale Gas Well Fracturing Fluids to Control Bacterially Relate
Available for download

08658 Use of Microbiocides in Barnett Shale Gas Well Fracturing Fluids to Control Bacterially Related Problems

Product Number: 51300-08658-SG
ISBN: 08658 2008 CP
Author: Jennifer K. Fichter, Kelly Johnson, Karl D. French, and Rod Owen
Publication Date: 2008
$20.00
Picture for Determining Biocide Selection and Dosage Recommendation via Planktonic and Sessile Kill Studies and
Available for download

51317--9802-Determining Biocide Selection and Dosage Recommendation via Planktonic and Sessile Kill Studies and Subsequent Biofilm Regrowth: A Case Study

Product Number: 51317--9802-SG
ISBN: 9802 2017 CP
Author: Zach Broussard
Publication Date: 2017
$20.00

A three-phase production system in the North Sea was experiencing multiple leaks in the topside separation facilities. To control the microbial contamination in the system, an initial planktonic kill study was performed to select the best possible biocides to provide immediate microbial mitigation to the field.

 
Categories
Industry
Recently viewed
Popular tags
View all

Association for Materials Protection and Performance

© AMPP All Rights Reserved.
Membership Terms of Content Use
Contact Customer Support