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Sometime More Is Just More: THPS Biocide Laboratory Kill Study on Wildtype Sulfate Reducing Bacteria.

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Tetrakis(hydroxymethyl)-phosphonium Sulfate (THPS) is a very common active ingredient in oil and gas biocides. While product labels provide broad guidelines application dosing the lowest effective dose of THPS is difficult to determine. Site water chemistry and bacteria biology variability will affect the dose need to achieve the desired level of bacteria population control. For these reasons biocide dose response studies are commonly conducted on solutions containing bacteria to determine the effect of treatments before application.

Product Number: 51323-18858-SG
Author: J. Fajt
Publication Date: 2023
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Biocide dose response studies are commonly conducted on water solutions containing bacteria to determine the effect of chemical treatments before application. Biocide product labels provide broad guidelines for dosing. However, site water chemistry and bacteria biology make the minimum effective dose differ for each location difficult to determine. A large volume culture of sulfate reducing bacteria (SRB) was prepared and allowed to grow until 4 log bacteria were present. The sample as then split into four identical 500 ml samples. The four samples were dosed at 0, 5, 50 and 400 ppm of tetrakis(hydroxymethyl)-phosphonium sulfate (THPS) based biocide. The effect on bacteria levels were tested using an enzyme-based bacteria metabolism test after 0.2, 1, 8, 24 and 96 hr. This study showed that a single application of 50 ppm of biocide could be as effective as a 400 ppm on high numbers of planktonic SRB.

Biocide dose response studies are commonly conducted on water solutions containing bacteria to determine the effect of chemical treatments before application. Biocide product labels provide broad guidelines for dosing. However, site water chemistry and bacteria biology make the minimum effective dose differ for each location difficult to determine. A large volume culture of sulfate reducing bacteria (SRB) was prepared and allowed to grow until 4 log bacteria were present. The sample as then split into four identical 500 ml samples. The four samples were dosed at 0, 5, 50 and 400 ppm of tetrakis(hydroxymethyl)-phosphonium sulfate (THPS) based biocide. The effect on bacteria levels were tested using an enzyme-based bacteria metabolism test after 0.2, 1, 8, 24 and 96 hr. This study showed that a single application of 50 ppm of biocide could be as effective as a 400 ppm on high numbers of planktonic SRB.

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