51312-01758-Novel DNA Extraction and Preservation for Identification of Micro-organisms On-site using Novel Nucl

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Product Number: 51312-01758-SG

ISBN: 01758 2012 CP

Author: Andrew C. Price

Publication Date: 2012

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Traditional microbiological methods based on culturing procedures such as the Most Probable Number (MPN) technique have been used for many years to monitor microbial numbers in oilfield samples. However such methods are far from ideal as it is estimated that at most than 15% of all microorganisms present in environmental samples are culturable by these methods. In addition without further extensive procedures being carried out such methods cannot identify the variety of microorganisms in the sample. Molecular biological methods negate such problems providing information on all the microorganisms present in an environmental sample. However traditional molecular biological methods do have their drawbacks particularly if employed on offshore oil installations. Such problems include microbial population changes occurring in environmental samples on transportation from the installation to the testing laboratory; additionally the transportation and use of hazardous chemicals employed in traditional DNA extraction procedures limit the use of these methods on offshore installations.One new technology extensively tested by Oil Plus the Whatman FTA system offers a simple and convenient solution to sample transport and on-site DNA extraction problems. The cards are impregnated with chemicals that lyse the cells and preserve the extracted DNA thus eliminating the need for hazardous DNA extraction / purification chemicals. Hence the cards ‘fix’ the DNA onto the card so the microbial population at the point of sampling is preserved eradicating concerns of changes in microbial populations occurring during sample transport. The presentation will discuss the mode of operation of the FTA technology trials with the FTA cards on oilfield samples comparisons with traditional DNA extraction procedures and recent developments with the technology. The talk will conclude with examples of how FTA technology has been used to develop proactive solutions to microbiological problems in the oil & gas industry.