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Impact Of Preservation Method, Storage Temperature, And Processing Time On Microbiological Community Shift In Solid Samples Collected From Crude Oil Transmission Pipelines

Impact of Preservation Method, Storage Temperature, and Processing Time on Microbiological Community Shift in Solid Samples Collected from Crude Oil Transmission Pipelines
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Robust integrity management plans are critical for ensuring the lifespan and preventing failures of manmade infrastructure, including the metal (carbon steel) infrastructure that dominates the oil and gas industry. In this sector and others, many types of corrosion can occur on metal infrastructure, including corrosion that involves the participation of microorganisms, commonly referred to as microbiologically influenced corrosion, or MIC. MIC can be difficult to diagnose as the cause of a given infrastructure failure because it is not a stand-alone mechanism – the physical and chemical properties of a system can influence the types of microorganisms that are present and active, while the metabolisms of these microorganisms can influence the surrounding chemistry and physical properties of a system.

Product Number: 51322-18009-SG
Author: Mohita Sharma, Nicole Taylor, Lisa Gieg, Trevor Place, Yin Shen, Danika Nicoletti, Jennifer Sargent
Publication Date: 2022
Industries: Oil and Gas , Coatings
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Microbiological monitoring of samples collected from oilfield operations is crucial for understanding microbial impacts on metal infrastructure. As many oilfields and related infrastructure are in remote locations, the time between sample collection, shipping to laboratory and sample processing for microbiological analysis can vary from days to weeks. Hence, robust sample handling and preservation methodology to arrest microbiological activity at the time of sample collection is needed.  To identify the best preservation methodologies that can be adopted for solids, oily sludge samples were collected twice from the nose of the same pig trap during a routine pipeline pigging operation of a crude oil transmission pipeline over a span of two years. Samples were chemically preserved at the time of sample collection (with different alcohols or commercially available nucleic acid preservation reagents) or remained unpreserved, and all samples were stored either at 4°C or at room temperature following collection. Samples were assessed using 16S rRNA gene sequencing to determine microbial community composition at day 0 (time of sample collection) and at different intervals for up to 50 days of storage, and comparisons in microbial community composition were made to the day 0 results.  

Microbiological monitoring of samples collected from oilfield operations is crucial for understanding microbial impacts on metal infrastructure. As many oilfields and related infrastructure are in remote locations, the time between sample collection, shipping to laboratory and sample processing for microbiological analysis can vary from days to weeks. Hence, robust sample handling and preservation methodology to arrest microbiological activity at the time of sample collection is needed.  To identify the best preservation methodologies that can be adopted for solids, oily sludge samples were collected twice from the nose of the same pig trap during a routine pipeline pigging operation of a crude oil transmission pipeline over a span of two years. Samples were chemically preserved at the time of sample collection (with different alcohols or commercially available nucleic acid preservation reagents) or remained unpreserved, and all samples were stored either at 4°C or at room temperature following collection. Samples were assessed using 16S rRNA gene sequencing to determine microbial community composition at day 0 (time of sample collection) and at different intervals for up to 50 days of storage, and comparisons in microbial community composition were made to the day 0 results.  

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