Menu
Search
Filters
Close
Menu

51315-5686-Field Applications for On-Site DNA Extraction and qPCR

Picture for Field Applications for On-Site DNA Extraction and qPCR
Available for download
Product Number: 51315-5686-SG
ISBN: 5686 2015 CP
Author: Crystal Lee
Publication Date: 2015
$0.00
$20.00
$20.00
Microbial issues have always been a problem in many areas of the petroleum industry. Microorganisms can cause concerns with fouling plugging and corrosion. One aspect of designing an effective integrity management program involves regular microbial monitoring and selection of the best chemistry for controlling microbes in a system. A variety of monitoring techniques are available that can be divided into two categories: on-site and off-site. Common practices for on-site bacterial monitoring include culture media bottles (i.e. serial dilution) and adenosine triphosphate (ATP) testing both of which have advantages and limitations. Serial dilution bottles can detect specific categories of bacteria such as sulfate-reducing bacteria but require 14 to 28 days for results. ATP testing is much faster requiring only minutes to process samples but is not specific in its detection of bacteria categories. The methods are also not as adept at identifying problematic archaea that may be present.To provide additional tools for monitoring microbes the oil field industry has begun using DNA detection for the presence of prokaryotes using methods such as quantitative polymerase chain reaction (qPCR) among others. DNA testing can provide improved sensitivity and specific information on the prokaryote of interest. Until recently the technique has not been available as an on-site analysis. Microbial samples were sent to specific testing locations causing long delays. The integrity of the samples can be compromised during the delay. The paper presents applications in oil field production incorporating the stages for processing DNA such as stabilization extraction and performing qPCR. The applications for the methods are useful for optimizing a microbial control program in addition to taking only a few days on-site to process as opposed to several weeks off-site. The novel on-site detection method is a useful tool in a broad range of applications for integrity management.
Microbial issues have always been a problem in many areas of the petroleum industry. Microorganisms can cause concerns with fouling plugging and corrosion. One aspect of designing an effective integrity management program involves regular microbial monitoring and selection of the best chemistry for controlling microbes in a system. A variety of monitoring techniques are available that can be divided into two categories: on-site and off-site. Common practices for on-site bacterial monitoring include culture media bottles (i.e. serial dilution) and adenosine triphosphate (ATP) testing both of which have advantages and limitations. Serial dilution bottles can detect specific categories of bacteria such as sulfate-reducing bacteria but require 14 to 28 days for results. ATP testing is much faster requiring only minutes to process samples but is not specific in its detection of bacteria categories. The methods are also not as adept at identifying problematic archaea that may be present.To provide additional tools for monitoring microbes the oil field industry has begun using DNA detection for the presence of prokaryotes using methods such as quantitative polymerase chain reaction (qPCR) among others. DNA testing can provide improved sensitivity and specific information on the prokaryote of interest. Until recently the technique has not been available as an on-site analysis. Microbial samples were sent to specific testing locations causing long delays. The integrity of the samples can be compromised during the delay. The paper presents applications in oil field production incorporating the stages for processing DNA such as stabilization extraction and performing qPCR. The applications for the methods are useful for optimizing a microbial control program in addition to taking only a few days on-site to process as opposed to several weeks off-site. The novel on-site detection method is a useful tool in a broad range of applications for integrity management.
Also Purchased
Picture for Development and Validation of In-Field qPCR Methods for Microbial Analysis at Oil and Gas Facilities
Available for download

Development and Validation of In-Field qPCR Methods for Microbial Analysis at Oil and Gas Facilities

Product Number: 51315-5948-SG
ISBN: 5948 2015 CP
Author: Neil Sharma
Publication Date: 2015
$0.00
Picture for Quantifying Sulfur Oxidizing Bacteria using qPCR
Available for download

51318-10902-Quantifying Sulfur Oxidizing Bacteria using qPCR

Product Number: 51318-10902-SG
Author: Scott Leleika / Amanda Harmon / Sarah Eisenlord
Publication Date: 2018
$20.00

We have developed a quantitative polymerase chain reaction (qPCR) assay to detect and quantify sulfur oxidizing bacteria (SOB) through the amplification of the soxB subunit of the thiosulfate-oxidizing gene complex. SOB populations have been linked to the corrosion of concrete and steel.
Picture for Quantifying the Risk of MIC
Available for download

51316-7347-Quantifying the Risk of MIC

Product Number: 51316-7347-SG
ISBN: 7347 2016 CP
Author: sarah eisenlord
Publication Date: 2016
$20.00

We describe the advancement of an activity-based quantitative polymerase chain reaction (qPCR) assay which can distinguish live from dead corrosion influencing microorganisms in oil and gas pipeline environments. We discuss the limitations and possible future optimization methods for Propidium monazide-qPCR techniques in the industry.
Categories
Industry
Recently viewed
Popular tags
View all

Association for Materials Protection and Performance

© AMPP All Rights Reserved.
Membership Terms of Content Use
Contact Customer Support