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Development and Validation of In-Field qPCR Methods for Microbial Analysis at Oil and Gas Facilities

Picture for Development and Validation of In-Field qPCR Methods for Microbial Analysis at Oil and Gas Facilities
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Product Number: 51315-5948-SG
ISBN: 5948 2015 CP
Author: Neil Sharma
Publication Date: 2015
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Multiple families of microorganisms have been implicated in Microbiologically Influenced Corrosion (MIC) within the oil and gas industry as well as in other fields resulting in billions of dollars in damage annually. The ability to detect these organisms in a timely and accurate manner can allow for the implementation of appropriate treatment regimens to mitigate costs and downtime. Unfortunately most current methods are either non-specific or require multiple days or weeks to generate actionable results.A fast and highly specific method quantitative polymerase chain reaction (qPCR) has begun to gain a foothold in the industry over the last several years. Current implementations however still require offsite processing of the sample and analysis which can take several days or even weeks due to shipping and other delays. During this time the microbial composition in the sample can also change limiting the reliability of the results.Our recent work has focused on developing and validating methods for direct in-field processing and qPCR analysis of water samples collected from oil and gas facilities using an easily transportable testing system packaged in a Pelican case. We report results encompassing the development of in-field methods for sample processing and nucleic acid extraction from water samples the design and validation of novel qPCR assays targeting specific microbial families such as sulfate reducing bacteria (SRB) and analysis using a portable qPCR instrument. The ability to conduct analyses in the field provides microbiologists with accurate and highly actionable results in just a few hours versus having to wait days or weeks.
Multiple families of microorganisms have been implicated in Microbiologically Influenced Corrosion (MIC) within the oil and gas industry as well as in other fields resulting in billions of dollars in damage annually. The ability to detect these organisms in a timely and accurate manner can allow for the implementation of appropriate treatment regimens to mitigate costs and downtime. Unfortunately most current methods are either non-specific or require multiple days or weeks to generate actionable results.A fast and highly specific method quantitative polymerase chain reaction (qPCR) has begun to gain a foothold in the industry over the last several years. Current implementations however still require offsite processing of the sample and analysis which can take several days or even weeks due to shipping and other delays. During this time the microbial composition in the sample can also change limiting the reliability of the results.Our recent work has focused on developing and validating methods for direct in-field processing and qPCR analysis of water samples collected from oil and gas facilities using an easily transportable testing system packaged in a Pelican case. We report results encompassing the development of in-field methods for sample processing and nucleic acid extraction from water samples the design and validation of novel qPCR assays targeting specific microbial families such as sulfate reducing bacteria (SRB) and analysis using a portable qPCR instrument. The ability to conduct analyses in the field provides microbiologists with accurate and highly actionable results in just a few hours versus having to wait days or weeks.
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