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Endospore Production in Response to Microbiocides in the Microbiologically Influenced Corrosion Implicated Genus Clostridium

Endospore-producing bacteria are often detected in oilfield produced waters via 16S rRNA identification. Although prevalent in oilfield brines little is known about their significance and potential implications on microbial control efforts. The presence of noxious chemicals and subsequent chemical damage can force a capable cell into an endospore a dormant structure that preserves the cellular genetic material. As extracellular conditions improve such as the dilution or removal of antimicrobials the endospore again becomes a vegetative cell. These endospores are largely impervious to and are not readily eradicated by antimicrobial agents. Here commonly used microbiocide chemistries are applied in differing concentrations and contact times in order to evaluate the cellular response scored as cell survival endospore formation or cell death. Outcomes were determined utilizing the schaeffer-fulton method for endospore staining and a fluorescence assay of bacterial viability based upon membrane integrity. Treatments are applied to cultured Bacillus subtilis the laboratory model organism commonly utilized to study endospore formation as well as unadulterated produced water containing members of the Phylum Firmicutes including the endospore capable Geobacillus stearothermophilus. Results indicate that the presence of a large population of endospore-forming bacteria may explain the failure of some microbiocide applications.

Product Number: 51319-13126-SG
Author: Jodi Wrangham
Publication Date: 2019
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