10408 Utilization of a 16S RRNA Gene Microarray (Phylochip) to Analyze the Efficacy of Oil and Gas Industry Bacteria Culture Media

Available for download

Product Number: 51300-10408-SG

ISBN: 10408 2010 CP

Author: Jodi Wrangham, Joe Penkala, Brent Peyton, Seth D'Imperio and Kenneth Wunch

Publication Date: 2010

Member Price: $0.00

Non Member Price: $20.00

Price: $20.00

Qty:

It is widely recognized that bacteria and archae are frequently contained within production fluids and can cause numerous problems and cost countless dollars to the oil and gas industries. Current techniques commonly employed to detect and enumerate these microorganisms and to test the efficacy of microbiocides focus on serial dilution culture methods. Unfortunately, culture-dependent methods allow for the growth of only a fraction of the field population present and do not support the growth of numerous potentially significant species. Currently, it is estimated that less than 15% of field populations can be cultivated by these means.

In this study, genetic profile analyses of microbial communities present in produced water samples were compared to those that were derived from culturing that produced water using various commonly used growth media. Genetic analyses utilized a 16S rRNA gene microarray which can detect 8,741 OTUs (Operational Taxonomic Units) in a single test. As expected and in congruence with samples from other environments, cultivation-dependent techniques greatly underestimated the microbial diversity present. These results illustrate one of the fundamental problems of culturedependent techniques: the large discrepancy between the microorganism population that is cultivated and detected in culture mediums and the population that is actually present in the oil and gas field environment.

KEYWORDS: Microbiologically-induced corrosion (MIC), Serial Dilution Culture Methods, Genetic Profile Analysis, 16S rRNA Gene Microarray