08653 Gene Array Analysis of Sulfate-Reducing Bacteria Grown on an Iron Electrode Under Conditions of Cathodic Protection

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Product Number: 51300-08653-SG

ISBN: 08653 2008 CP

Author: Gerrit Voordouw, Sean Caffrey, Hyung-Soo Park, and Jenny Been

Publication Date: 2008

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Sulfate-reducing bacteria (SRB) can contribute to microbially-influenced corrosion (MIC) of iron. Removal of hydrogen formed at the metal surface is often regarded as a critical step in the progression of MIC. Hence SRB-mediated iron corrosion and hydrogen oxidation may be comparable processes. Because the genome sequence of the model SRB Desulfovibrio vulgaris Hildenborough (DvH) is now known, this theory can be tested by genomics technologies. D. vulgaris has four periplasmic hydrogenases to oxidize molecular hydrogen, one iron-only (Hyd), two nickel-iron (Hyn1 and Hyn2) and one nickel-iron-selenium (Hys) hydrogenase. Total RNA was extracted from lactategrown cells, from 5%- or 50%-hydrogen-grown cells and from cells grown under conditions of cathodic protection. The lactate- and hydrogen-grown cells were planktonic, whereas the cathodic-protectiongrown cells were attached to the iron electrode. Genome-wide gene expression profiles were obtained by hybridizing labelled cDNA made from the extracted RNA preparations with microarrays containing a 70-mer oligonucleotide probe for each of the approximately 3000 genes from DvH.