Evaluation of a Self-Contained Anaerobic Environment-Generating Culture Device for Detection of Sulfate-Reducing Bacteria

Certain classes of microorganisms (e.g. sulfate reducing bacteria or SRB) have been linked to an increased risk of microbiologically influenced corrosion (MIC) and reservoir souring. The ability to quickly and accurately detect and enumerate MIC associated microbes is an essential part of an informed and efficacious mitigation strategy. Despite advances in molecular microbiology methods (MMMs) and adenosine triphosphate (ATP) detection culture based detection remains a key aspect of industrial and oil field microbiology. Detection and enumeration of MIC causing microbes in the field continues to be a challenge as they can be difficult to grow using traditional microbiological methods (e.g anaerobic culture bottles). Here we evaluate a thin-film culture device method for growth based detection of SRBs in surface flowback cooling and produced waters. Evaluation using laboratory strains as well as field samples was performed and compared to industry standard culture practices. In addition the thin-film culture devices were used to test waters pre- and post-biocide treatment to quickly assess biocide efficacy. The thin-film culture device technology was fast easy to use and recovered a wider variety of strains than traditional tests.

Product Number: 51319-13174-SG
Author: Evan Brutinel
Publication Date: 2019
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51312-01126-Correlation Between the Growth of a Mixed Culture of Sulphate-Reducing Bacteria Isolated from Produc

Product Number: 51312-01126-SG
ISBN: 01126 2012 CP
Author: Antonio J. De Turris
Publication Date: 2012