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97205 THE EFFECT OF GLUTARALDEHYDE ON THE DEVELOPMENT OF MARINE BIOFILMS FORMED ON SURFACES OF AISI 304 STAINLESS STEEL

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Product Number: 51300-97205-SG
ISBN: 97205 1997 CP
Author: R.C. Tapper, J.R. Smith, LB. Beech, M.R. Viera, P.S. Guimtret, H. Videla, C.L. Swords, R.G.J. Edyvea
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The effect of pre-conditioning polished and unpolished AISI 304 stsinless steel surfaces with glutamldehyde on the attachment, growth and morphology of an aerobic consortium of marine bacteria was investigated using total cell number counts, epifluorescence microscopy, Atomic Force Microscopy (AFl) Enviromnental Scanning Electron Microscopy (ESEM) and grazing-angle Fourier Transform Infrared (FTIR) spectroscopy. Both fully hydrated and dehydrated biofilms were studied using AFM and ESEM. Formation of the conditioning layer on steel surfaces from the culture medium, in the presence and absence of glutamhlehyde was monitored in-situ employing AFM and Grazing Angle FITR spectroscopy. The influence of both surface area and surface energy upon the numbers of bacteria attached to polished and repolished coupons was determined. This study has shown the influence of pretreatment of AHI 304 stainless steel with glutaraldehyde upon biofilm formtion and has demonstrated the ability of AFM, ESEM and FTIR to be used as valuable tools for the in-situ investigation of the effect of biocides on bacterial biofilms. Keywords: atomic force microscopy, bacterial attachment, bacterial consortia, biocides, enviromnental scanning electron microscopy, glutaraldehyde, stainless steel, marine biofilms.
The effect of pre-conditioning polished and unpolished AISI 304 stsinless steel surfaces with glutamldehyde on the attachment, growth and morphology of an aerobic consortium of marine bacteria was investigated using total cell number counts, epifluorescence microscopy, Atomic Force Microscopy (AFl) Enviromnental Scanning Electron Microscopy (ESEM) and grazing-angle Fourier Transform Infrared (FTIR) spectroscopy. Both fully hydrated and dehydrated biofilms were studied using AFM and ESEM. Formation of the conditioning layer on steel surfaces from the culture medium, in the presence and absence of glutamhlehyde was monitored in-situ employing AFM and Grazing Angle FITR spectroscopy. The influence of both surface area and surface energy upon the numbers of bacteria attached to polished and repolished coupons was determined. This study has shown the influence of pretreatment of AHI 304 stainless steel with glutaraldehyde upon biofilm formtion and has demonstrated the ability of AFM, ESEM and FTIR to be used as valuable tools for the in-situ investigation of the effect of biocides on bacterial biofilms. Keywords: atomic force microscopy, bacterial attachment, bacterial consortia, biocides, enviromnental scanning electron microscopy, glutaraldehyde, stainless steel, marine biofilms.
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